HPLC (High-Performance Liquid Chromatography) is the gold standard for peptide purity verification. Learn what it measures, why 99%+ matters, and how to read a COA.
High-Performance Liquid Chromatography (HPLC) is a laboratory technique that separates, identifies, and quantifies components within a mixture. For peptide quality control, HPLC is the gold standard for purity determination.
HPLC forces the sample through a column packed with a stationary phase material under high pressure. Different compounds travel through the column at different rates based on their chemical properties, producing peaks on a chromatogram. The area under each peak corresponds to the percentage of that component in the sample.
A peptide with 99%+ HPLC purity means:
This is the standard required for research-grade peptides. Lower purity peptides contain higher levels of impurities that may interfere with research results or introduce confounding variables.
A complete COA for a research peptide should include:
| Element | What to Look For |
|---|---|
| HPLC Chromatogram | Single dominant peak, minimal impurity peaks |
| Purity % | ≥99% for research grade |
| Retention time | Matches reference standard |
| Mass Spectrometry (MS) | Molecular weight matches theoretical |
| Batch Number | Traceable to production records |
| Endotoxin (LAL) | <1.0 EU/mg |
| Sterility | Pass |
Some suppliers perform in-house testing, which creates a conflict of interest. At Peptide Warehouse Australia, every batch is tested by an independent, accredited third-party laboratory. This ensures:
1. Batch synthesis by GMP-certified manufacturer
2. Sample sent to independent Australian laboratory
3. HPLC purity analysis and chromatogram generation
4. Mass spectrometry identity confirmation
5. LAL endotoxin assay (target: <1.0 EU/mg)
6. Sterility testing
7. COA issued and published in our COA Library
Only batches passing all criteria are dispatched.
Disclaimer: For educational purposes related to in-vitro research quality standards. Not medical advice.
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99%+ HPLC purity · COA verified every batch · Next-day dispatch from Melbourne
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